Molecular characterisation and antibiotic sensitivity profile of Pasteurella multocida isolated from poultry farms in Malaysia

نویسندگان

چکیده

Fowl cholera has caused significant economic losses in many poultry producing countries worldwide. In Malaysia, outbreaks of fowl are frequently reported and encountered different types productions. The objective this study was to characterise 13 avian Pasteurella multocida, isolated from Central Peninsular Malaysia the period between 2000 2018. isolates were subjected multiplex polymerase chain reaction (PCR) for capsular serotyping, disc diffusion method antimicrobial susceptibility profiles, molecular genotyping using pulsed-field gel electrophoresis (PFGE) multilocus sequence typing (MLST). serotyping showed all multocida belonging serotype A. several multidrug resistance strains among P. isolates. All resistant erythromycin (100%), streptomycin (68%), tetracycline (37%), enrofloxacin florfenicol (23%), penicillin G (14%), gentamicin amoxicillin (14%). PFGE analysis clustered into three clones. Group A included with a similarity 87% year 2000, 2013, Three identified MLST namely, ST129, ST231, ST355. ST355 assigned first time Rural Industries Research Development Corporation (RIRDC) database. Besides, ST129 been India, China, Sri Lanka, which highlights possibility transmission Asian countries. This provides an insight epidemiological information that causes central region Malaysia.

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ژورنال

عنوان ژورنال: Austral journal of veterinary sciences

سال: 2021

ISSN: ['0719-8000', '0719-8132']

DOI: https://doi.org/10.4067/s0719-81322021000200121